WARS2 Polyclonal antibody

WARS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13944-1-AP

Synonyms

(Mt)TrpRS, TrpRS, Tryptophan tRNA ligase, WARS2

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Validation Data Gallery

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  WB analysis of human liver using 13944-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13944-1-AP (WARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human liver tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13944-1-AP targets WARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WARS2 fusion protein Ag5024
• Full Name: tryptophanyl tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 60 kDa/55 kDa
• GenBank Accession Number: BC044575
• Gene Symbol: WARS2
• Gene ID (NCBI): 10352
• RRID: AB_2304541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WARS2(tryptophan--tRNA ligase, mitochondrial) is also called mitochondrial TRPRS and belongs to the class-I aminoacyl-tRNA synthetase family. The gene encodes the protein containing 360-amino acid ,which has an N-terminal mitochondrial targeting signal that is cleaved after residue 18. WARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold(PMID:15779907). It has 2 isoforms produced by alternative splicing.