UQCC2 Polyclonal antibody

UQCC2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25781-1-AP

Synonyms

C6orf125, uqcc2

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Validation Data Gallery

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  WB analysis of HEK-293 using 25781-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25781-1-AP (UQCC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25781-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25781-1-AP (UQCC2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25781-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25781-1-AP (UQCC2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25781-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25781-1-AP (UQCC2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 25781-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25781-1-AP (UQCC2 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25781-1-AP

  Immunofluorescent analysis of HepG2 cells using 25781-1-AP (UQCC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 25781-1-AP is UQCC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UQCC2 fusion protein Ag22710
• Full Name: chromosome 6 open reading frame 125
• Calculated Molecular Weight: 126 aa, 15 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC006007
• Gene Symbol: C6orf125
• Gene ID (NCBI): 84300
• RRID: AB_2880237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCC2, also named as C6orf125, MNF1, Mitochondrial protein M19 and Breast cancer-associated protein SGA-81M, plays a role in the modulation of respiratory chain activities. It's a mitochondrial protein.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Combined Respiratory Chain Deficiency and UQCC2 Mutations in Neonatal Encephalomyopathy: Defective Supercomplex Assembly in Complex III Deficiencies.; Authors - René G Feichtinger
mouse	WB	Cell Rep; A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression.; Authors - Evanthia Pangou
human	WB	iScience; Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis; Authors - Yunfan He