UAP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

UAP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2C7B2

Cat no : 67545-1-Ig

Synonyms

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

---

Validation Data Gallery

• 

  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 67545-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, NCI-H1299 cells, LNCaP cells, HepG2 cells, THP-1 cells, HEK-293 cells, DU 145 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

67545-1-Ig targets UAP1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UAP1 fusion protein Ag9513
• Full Name: UDP-N-acteylglucosamine pyrophosphorylase 1
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 57-59 kDa
• GenBank Accession Number: BC009377
• Gene Symbol: UAP1
• Gene ID (NCBI): 6675
• RRID: AB_2882762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

Publications

Species	Application	Title
human	WB,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Mol Cell; Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response; Authors - Shuai Yang ; KD Validated
human	IHC,WB	Front Immunol; The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma; Authors - Zexin Su