TFIP11 Polyclonal antibody

TFIP11 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14436-1-AP

Synonyms

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

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Validation Data Gallery

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  WB analysis of HeLa using 14436-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14436-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14436-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14436-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14436-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14436-1-AP (TFIP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14436-1-AP targets TFIP11 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFIP11 fusion protein Ag5821
• Full Name: tuftelin interacting protein 11
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC033080
• Gene Symbol: TFIP11
• Gene ID (NCBI): 24144
• RRID: AB_11182269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.