CoraLite® Plus 488-conjugated TFF2 Polyclonal antibody

TFF2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13681

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2

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Validation Data Gallery

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  IF Staining of mouse stomach using CL488-13681

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using CoraLite® Plus 488 TFF2 antibody (CL488-13681) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse stomach tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13681 targets TFF2 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFF2 fusion protein Ag4537
• Full Name: trefoil factor 2
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC032820
• Gene Symbol: TFF2
• Gene ID (NCBI): 7032
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).