TFB2M Polyclonal antibody

• Featured Product
• KD/KO Validated

TFB2M Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24411-1-AP

Synonyms

h mtTFB, h mtTFB2, Hkp1, hTFB2M, mtTFB2, NS5ATP5, TFB2M

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Validation Data Gallery

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  WB analysis of mouse testis using 24411-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24411-1-AP (TFB2M Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24411-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24411-1-AP (TFB2M Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

24411-1-AP targets TFB2M in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TFB2M fusion protein Ag19687
• Full Name: transcription factor B2, mitochondrial
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC003383
• Gene Symbol: TFB2M
• Gene ID (NCBI): 64216
• RRID: AB_2879530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFB2M (mitochondrial transcription factor B2) is a novel nuclear-encoded protein that is necessary for transcription of mitochondrial DNA.

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	WB	iScience; MUC1-C integrates aerobic glycolysis with suppression of oxidative phosphorylation in triple-negative breast cancer stem cells; Authors - Nami Yamashita
human	WB	Cells; A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication; Authors - Natalya Kozhukhar ; KO Validated