TEX15 Polyclonal antibody

TEX15 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24585-1-AP

Synonyms

Cancer/testis antigen 42, CT42, testis expressed 15, TEX15

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Validation Data Gallery

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  WB analysis of rat testis using 24585-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24585-1-AP (TEX15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 24585-1-AP

  IP result of anti-TEX15 (IP:24585-1-AP, 4ug; Detection:24585-1-AP 1:300) with PC-3 cells lysate 3200ug.

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  IHC staining of mouse testis using 24585-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24585-1-AP (TEX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24585-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24585-1-AP (TEX15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat testis tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24585-1-AP is TEX15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX15 fusion protein Ag20170
• Full Name: testis expressed 15
• Calculated Molecular Weight: 2789 aa, 315 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC141841
• Gene Symbol: TEX15
• Gene ID (NCBI): 56154
• RRID: AB_2879623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX15 also named as Cancer/testis antigen 42 is a 2789 amino acid protein, which is expressed exclusively in testicular and cancerous tissues and may be involved in the development and metastasis of several different types of tumors. TEX15 exists as a 1279 amino acid protein in the mouse and rat testis. The molecular weight of mouse/rat TEX15 is 150 kDa (PMID: 26959739).

Publications

Species	Application	Title
mouse	WB	Toxicol Lett; Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium.; Authors - Yu Fang
human	WB	Fertil Steril; Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility.; Authors - Masood Abu-Halima