TEKT4 Polyclonal antibody

TEKT4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17058-1-AP

Synonyms

TEKT4, tektin 4

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 17058-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 17058-1-AP (TEKT4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17058-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17058-1-AP (TEKT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17058-1-AP

  IP result of anti-TEKT4 (IP:17058-1-AP, 4ug; Detection:17058-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human kidney using 17058-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17058-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17058-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, human testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human lung tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17058-1-AP targets TEKT4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEKT4 fusion protein Ag10772
• Full Name: tektin 4
• Calculated Molecular Weight: 435 aa, 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC021716
• Gene Symbol: TEKT4
• Gene ID (NCBI): 150483
• RRID: AB_2203334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF,CoIP	Nat Commun; Enriched variations in TEKT4 and breast cancer resistance to paclitaxel.; Authors - Yi-Zhou Jiang
mouse	WB,IP	Cell Mol Life Sci; Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella; Authors - Xin-Yan Geng