TDRD5 Polyclonal antibody

TDRD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25239-1-AP

Synonyms

RP11 427G13.1, TDRD5, tudor domain containing 5, TUDOR3

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Validation Data Gallery

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  WB analysis of mouse testis using 25239-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25239-1-AP (TDRD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 25239-1-AP

  IP result of anti-TDRD5 (IP:25239-1-AP, 5ug; Detection:25239-1-AP 1:300) with mouse testis tissue lysate 2800ug.

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  IHC staining of mouse testis using 25239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25239-1-AP targets TDRD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TDRD5 fusion protein Ag18896
• Full Name: tudor domain containing 5
• Calculated Molecular Weight: 981 aa, 110 kDa
• Observed Molecular Weight: ~116 kDa
• GenBank Accession Number: BC130532
• Gene Symbol: TDRD5
• Gene ID (NCBI): 163589
• RRID: AB_2879981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TDRD5, also named as Tudor domain-containing protein 5, is a 981 amino acid protein, which contains one Tudor domain and three HTH OST-type domains. TDRD5 localizes in the cytiplasm and belongs to the TDRD5 family. TDRD5 is required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity.