TBX4/5 Polyclonal antibody

TBX4/5 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 13178-1-AP

Synonyms

HOS, T box 5, T box protein 5, TBX5

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Validation Data Gallery

• 

  WB analysis of HeLa using 13178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13178-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13178-1-AP (TBX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13178-1-AP

  Immunofluorescent analysis of HepG2 cells using 13178-1-AP (TBX5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below

Product Information

13178-1-AP targets TBX4/5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBX4/5 fusion protein Ag3845
• Full Name: T-box 5
• Calculated Molecular Weight: 518 aa, 57 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC027942
• Gene Symbol: TBX5
• Gene ID (NCBI): 6910
• RRID: AB_10638435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.

Publications

Species	Application	Title
human	IF	Eur Respir J; Single-cell RNA sequencing reveals that BMPR2 mutation regulates right ventricular function via ID genes.; Authors - Mingxia Du
human	WB	Mol Biol Evol; Functional and Adaptive Significance of Promoter Mutations that Affect Divergent Myocardial Expressions of TRIM72 in Primates.; Authors - Yuanqing Feng
mouse	WB	Environ Sci Technol; Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.; Authors - Zhihua Ren
		Stem Cell Reports; Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures.; Authors - Shota Kurotsu
human	IF	iScience; A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells.; Authors - Zaniar Ghazizadeh
mouse	WB	Sci Rep; Stoichiometric optimization of Gata4, Hand2, Mef2c, and Tbx5 expression for contractile cardiomyocyte reprogramming.; Authors - Zhentao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Young-Jae (Verified Customer) (12-06-2018)

Working good for western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: fibroblasts overexpressed with Tbx5