TBX15 Polyclonal antibody

TBX15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25148-1-AP

Synonyms

T box 15, TBX14, TBX15

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Validation Data Gallery

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  WB analysis using 25148-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25148-1-AP (TBX15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25148-1-AP is TBX15 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBX15 fusion protein Ag18423
• Full Name: T-box 15
• Calculated Molecular Weight: 602 aa, 66 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC122553
• Gene Symbol: TBX15
• Gene ID (NCBI): 6913
• RRID: AB_2879924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX15 is a transcription factor from the T-box family and is a highly pleiotropic gene expressed in multiple tissues at different stages of development. It is required for skeletal development and deletions in this gene cause severe skeletal malformation. In addition, TBX15 also plays a role in the differentiation of brown and beige adipocytes (PMID: 28007980). The predicted molecular weight of TBX15 is 66 kDa. With modification, the MW of TBX15 will be migrated to 50 kDa.

Publications

Species	Application	Title
human,mouse	WB	iScience; TBX15 facilitates malignant progression of glioma by transcriptional activation of TXDNC5; Authors - Yuyuan Ge