TBPL1 Polyclonal antibody

TBPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 12258-1-AP

Synonyms

21 kDa TBP like protein, STUD, TBP like 1, TBP like factor, TBP like protein 1, TBP related factor 2, TBP related protein, TBPL1, TLF, TLP, TLP21, TRF2, TRP

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Validation Data Gallery

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  WB analysis of mouse testis using 12258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12258-1-AP (TBPL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12258-1-AP

  IP result of anti-TBPL1 (IP:12258-1-AP, 4ug; Detection:12258-1-AP 1:300) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human stomach cancer tissue, mouse testis tissue, mouse stomach tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

12258-1-AP targets TBPL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBPL1 fusion protein Ag2880
• Full Name: TBP-like 1
• Calculated Molecular Weight: 186 aa, 21 kDa
• Observed Molecular Weight: 21 kDa, 25 kDa
• GenBank Accession Number: BC017559
• Gene Symbol: TBPL1
• Gene ID (NCBI): 9519
• RRID: AB_10642841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Cell; A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.; Authors - Peng Dai
mouse	WB	Pharmacol Res; Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway; Authors - Xiaohua Xie
human	WB	Cell Signal; C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1.; Authors - Hui Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jianing (Verified Customer) (09-03-2019)

This antibody recognize human tbpl1 strongly which give a nice clear band. But the efficiency against zebrafish tbpl1 is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell culture lysate