TBL1X Monoclonal antibody

TBL1X Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1A9A1

Cat no : 66955-1-Ig

Synonyms

EBI, SMAP55, TBL1, TBL1X

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 66955-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66955-1-Ig (TBL1X antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66955-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66955-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66955-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66955-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 66955-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TBL1X antibody (66955-1-Ig, Clone: 1A9A1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:100000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66955-1-Ig targets TBL1X in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TBL1X fusion protein Ag5291
• Full Name: transducin (beta)-like 1X-linked
• Calculated Molecular Weight: 526 aa, 57 kDa
• Observed Molecular Weight: 57 kDa, 62 kDa
• GenBank Accession Number: BC032708
• Gene Symbol: TBL1X
• Gene ID (NCBI): 6907
• RRID: AB_2882278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human	WB	Arterioscler Thromb Vasc Biol; Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice; Authors - Mei Yang
human	WB	J Virol; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.; Authors - Wenxiao Gong