TADA2L Polyclonal antibody

TADA2L Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11299-1-AP

Synonyms

ADA2, ADA2 like protein, ADA2A, hADA2, KL04P, TADA2A, TADA2L, Transcriptional adapter 2 like

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Validation Data Gallery

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  WB analysis of mouse testis using 11299-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11299-1-AP (TADA2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11299-1-AP

  IP result of anti-TADA2L (IP:11299-1-AP, 4ug; Detection:11299-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11299-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11299-1-AP (TADA2L antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11299-1-AP targets TADA2L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TADA2L fusion protein Ag1817
• Full Name: transcriptional adaptor 2 (ADA2 homolog, yeast)-like
• Calculated Molecular Weight: 51 aa, 5 kDa
• Observed Molecular Weight: 51.5 kDa
• GenBank Accession Number: BC011753
• Gene Symbol: TADA2L
• Gene ID (NCBI): 6871
• RRID: AB_717042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TADA2A is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes [PMID:19103755].

Publications

Species	Application	Title
human	WB	Mol Cell Biol; The double-histone-acetyltransferase complex ATAC is essential for mammalian development.; Authors - Guelman Sebastián S