Synaptogyrin 1 Polyclonal antibody

Synaptogyrin 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29734-1-AP

Synonyms

synaptogyrin 1, SYNGR1

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Validation Data Gallery

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  WB analysis using 29734-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29734-1-AP

  Unboiled rat brain tissue was subjected to SDS PAGE followed by western blot with 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 29734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 29734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 29734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (Synaptogyrin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: unboiled rat brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29734-1-AP targets Synaptogyrin 1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Synaptogyrin 1 fusion protein Ag31214
• Full Name: synaptogyrin 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC000731
• Gene Symbol: SYNGR1
• Gene ID (NCBI): 9145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.