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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Spartin, SPG20 Polyclonal antibody

Spartin, SPG20 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13791

Synonyms

KIAA0610, SPARTIN, Spartin, SPG20, Spastic paraplegia 20 protein, SPG20, TAHCCP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated Spartin, SPG20 Polyc (CL647-13791)

    IF Staining of HepG2 using CL647-13791

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 647 Spartin, SPG20 antibody (CL647-13791) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated Spartin, SPG20 Polyc (CL647-13791)

    FC experiment of HepG2 using CL647-13791

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Spartin, SPG20 (CL647-13791) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13791 targets Spartin, SPG20 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Spartin, SPG20 fusion protein Ag4815
Full Name spastic paraplegia 20 (Troyer syndrome)
Calculated Molecular Weight 75 kDa, 85 kDa
Observed Molecular Weight 75 kDa, 84 kDa
GenBank Accession NumberBC047083
Gene Symbol SPG20
Gene ID (NCBI) 23111
RRIDAB_2920224
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Spartin, SPG20 antibody CL647-13791Download protocol
FC protocol for CL Plus 647 Spartin, SPG20 antibody CL647-13791Download protocol
Standard Protocols
Click here to view our Standard Protocols