Septin 9 Monoclonal antibody

Septin 9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6F10

Cat no : 67548-1-Ig

Synonyms

AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, Septin 9, Septin D1, SINT1

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Validation Data Gallery

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  WB analysis using 67548-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67548-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 67548-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig colon tissue, A549 cells, rat colon tissue, MCF-7 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67548-1-Ig targets Septin 9 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

• Tested Reactivity: Human, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Septin 9 fusion protein Ag18047
• Full Name: septin 9
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC021192
• Gene Symbol: SEPT9
• Gene ID (NCBI): 10801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).