• Featured Product
  • KD/KO Validated

SYTL4 Polyclonal antibody

SYTL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12128-1-AP

Synonyms

Exophilin 2, Granuphilin, SLP4, synaptotagmin like 4, Synaptotagmin like protein 4, SYTL4



Tested Applications

Positive WB detected inHeLa cells, mouse pancreas tissue, U2OS cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12128-1-AP targets SYTL4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYTL4 fusion protein Ag2772
Full Name synaptotagmin-like 4
Calculated Molecular Weight 671 aa, 76 kDa
Observed Molecular Weight76 kDa
GenBank Accession NumberBC014913
Gene Symbol SYTL4
Gene ID (NCBI) 94121
RRIDAB_2877828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)

Protocols

Product Specific Protocols
WB protocol for SYTL4 antibody 12128-1-APDownload protocol
IHC protocol for SYTL4 antibody 12128-1-APDownload protocol
IP protocol for SYTL4 antibody 12128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Authors - Akiko Takahashi
humanWB,IP

Arterioscler Thromb Vasc Biol

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.

Authors - Frédéric Adam
humanWB,IF

Theranostics

SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.

Authors - Xi-Yu Liu
  • KD Validated
humanWB

J Proteome Res

Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses.

Authors - Wu Hsin-Yi HY
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li