SYT7 Polyclonal antibody

SYT7 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19741-1-AP

Synonyms

IPCA 7, PCANAP7, Synaptotagmin 7, synaptotagmin VII, SYT VII, SYT7, SytVII

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Validation Data Gallery

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  WB analysis using 19741-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cerebellum using 19741-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IF detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19741-1-AP targets SYT7 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptotagmin VII
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_004200
• Gene Symbol: SYT7
• Gene ID (NCBI): 9066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca²⁺-dependent exocytosis of secretory vesicles through Ca²⁺ and phospholipid binding to the C2 domain or may serve as Ca²⁺ sensors in the process of vesicular trafficking and exocytosis.