SV2C Polyclonal antibody

SV2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21271-1-AP

Synonyms

KIAA1054, SV2C

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Validation Data Gallery

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  WB analysis of mouse brain using 21271-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 21271-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21271-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21271-1-AP

  IP result of anti-SV2C (IP:21271-1-AP, 4ug; Detection:21271-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of mouse cerebellum using 21271-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21271-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skin tissue, C6 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21271-1-AP is SV2C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SV2C fusion protein Ag15745
• Full Name: synaptic vesicle glycoprotein 2C
• Calculated Molecular Weight: 727 aa, 82 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC100826
• Gene Symbol: SV2C
• Gene ID (NCBI): 22987
• RRID: AB_10755297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.