CoraLite® Plus 488-conjugated SUFU Polyclonal antibody

• Featured Product
• KD/KO Validated

SUFU Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26759

Synonyms

PRO1280, SUFU, SUFUH, SUFUXL, Suppressor of fused homolog

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Validation Data Gallery

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  IF Staining of HeLa using CL488-26759

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SUFU antibody (CL488-26759) at dilution of 1:200.

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  FC experiment of HEK-293 using CL488-26759

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SUFU (CL488-26759) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26759 targets SUFU in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUFU fusion protein Ag25200
• Full Name: suppressor of fused homolog (Drosophila)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC013291
• Gene Symbol: SUFU
• Gene ID (NCBI): 51684
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sufu (Suppressor of fused) is a central regulator of Hh (Hedgehog) signaling and acts as a tumor suppressor by maintaining the Gli (Glioma-associated oncogene homolog) transcription factors inactive. Loss of Sufu function promotes tumorigenesis.