STXBP6 Polyclonal antibody

STXBP6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10976-4-AP

Synonyms

amisyn, HSPC156, STXBP6, Syntaxin binding protein 6

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Validation Data Gallery

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  WB analysis using 10976-4-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10976-4-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10976-4-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10976-4-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10976-4-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10976-4-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10976-4-AP (STXBP6 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human brain tissue, BxPC-3 cells, rat brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10976-4-AP targets STXBP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STXBP6 fusion protein Ag1427
• Full Name: syntaxin binding protein 6 (amisyn)
• Calculated Molecular Weight: 25 kDa or 45 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC009499
• Gene Symbol: STXBP6
• Gene ID (NCBI): 29091
• RRID: AB_2197002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.