STXBP4 Polyclonal antibody

STXBP4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14231-1-AP

Synonyms

STX4 interacting protein, STXBP4, Synip, Syntaxin 4 interacting protein, syntaxin binding protein 4

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Validation Data Gallery

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  WB analysis using 14231-1-AP

  PC-3 lysates were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14231-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14231-1-AP (STXBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14231-1-AP (STXBP4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of BxPC-3 using 14231-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14231-1-AP (STXBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, human heart tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14231-1-AP targets STXBP4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STXBP4 fusion protein Ag5471
• Full Name: syntaxin binding protein 4
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC041485
• Gene Symbol: STXBP4
• Gene ID (NCBI): 252983
• RRID: AB_2196980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.