STS Polyclonal antibody

• Featured Product
• KD/KO Validated

STS Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17870-1-AP

Synonyms

ARSC, ARSC1, Arylsulfatase C, ASC, ES, SSDD, Steroid sulfatase, Steryl sulfatase, Steryl sulfate sulfohydrolase, STS

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Validation Data Gallery

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  WB analysis of MCF-7 using 17870-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17870-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 17870-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17870-1-AP (STS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, human placenta tissue
• Positive IHC detected in: human placenta tissue, human hysteromyoma tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

17870-1-AP targets STS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STS fusion protein Ag12353
• Full Name: steroid sulfatase (microsomal), isozyme S
• Calculated Molecular Weight: 583 aa, 65 kDa
• Observed Molecular Weight: 63-65 kDa
• GenBank Accession Number: BC075030
• Gene Symbol: STS
• Gene ID (NCBI): 412
• RRID: AB_2240080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STS(Steroid sulfatase) is also named as ASC(arylsulfatase C), ARSC1 and belongs to the sulfatase family. It is expressed in human liver and is responsible for the hydrolysis of many estrogen and hydroxysteroid sulfates, including dehydroepiandrosterone (DHEA)-sulfate and β-estradiol(E2)-3-sulfate(PMID:19589875). This enzyme has also been documented to occur in many other tissues, including skin, lung, ovary, and adrenal gland. The estimated molecular mass of expressed human STS is 63-85 kDa which may represent different levels of glycosylation in the different tissues, as STS is known to be a glycoprotein and the full length protein has a signal peptide with 21 amino acids(PMID:17604157).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Steroid Sulfatase Stimulates Intracrine Androgen Synthesis and is a Therapeutic Target for Advanced Prostate Cancer.; Authors - Cameron M Armstrong ; KD Validated
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li
human	WB	Mol Cell Biochem; Resveratrol and its methoxy derivatives modulate the expression of estrogen metabolism enzymes in breast epithelial cells by AhR down-regulation.; Authors - Barbara Licznerska
mouse	WB,IF	J Biomed Res; Suppressed estrogen supply via extra-ovarian progesterone receptor membrane component 1 in menopause.; Authors - Sang R Lee
mouse	IF	Life Sci Alliance; Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration; Authors - Yafang Wang