STRN3 Polyclonal antibody

STRN3 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 23966-1-AP

Synonyms

Cell cycle autoantigen SG2NA, GS2NA, S/G2 antigen, SG2NA, Striatin 3, STRN3

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Validation Data Gallery

• 

  WB analysis using 23966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23966-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 23966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23966-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23966-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, MCF-7 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23966-1-AP is STRN3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STRN3 fusion protein Ag21091
• Full Name: striatin, calmodulin binding protein 3
• Calculated Molecular Weight: 797 aa, 87 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC126221
• Gene Symbol: STRN3
• Gene ID (NCBI): 29966
• RRID: AB_2879379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah
human	WB,IF	STAR Protoc; Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy; Authors - Wangsheng Ji
human	WB	Genes Genomics; LncRNA KTN1-AS1 facilitates esophageal squamous cell carcinoma progression via miR-885-5p/STRN3 axis; Authors - Liying Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works well with Western Blot but requires long exposure >100s. Some nonspecific band but still not dirty.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

STRN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:23966-1-AP)