STK33 Polyclonal antibody

STK33 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12857-1-AP

Synonyms

serine/threonine kinase 33, STK33

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Validation Data Gallery

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  WB analysis of human kidney using 12857-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12857-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12857-1-AP

  IP result of anti-STK33 (IP:12857-1-AP, 4ug; Detection:12857-1-AP 1:300) with HEK-293 cells lysate 880ug.

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  IHC staining of human liver cancer using 12857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 12857-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using STK33 antibody (12857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human kidney tissue, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12857-1-AP targets STK33 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK33 fusion protein Ag3561
• Full Name: serine/threonine kinase 33
• Calculated Molecular Weight: 514 aa, 58 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC031231
• Gene Symbol: STK33
• Gene ID (NCBI): 65975
• RRID: AB_10642563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine kinase 33 (STK33) gene, which is identified in human chromosome 11 and mouse chromosome 7, encodes a serine/threonine kinase. STK33 belongs to a member of the calcium/calmodulin‐dependent kinase (CAMK), which is mainly existed in testis, certain brain regions and embryonic organs such as brain, heart and spinal cord in human and mouse and is likely to participate in spermatogenesis and organ ontogenesis. (PMID: 30256516, PMID: 34536775)

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; STK33 phosphorylates fibrous sheath protein AKAP3/4 to regulate sperm flagella assembly in spermiogenesis; Authors - Weiling Yu