STK10 Polyclonal antibody

STK10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 25471-1-AP

Synonyms

LOK, Lymphocyte oriented kinase, PRO2729, serine/threonine kinase 10, STK10

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Validation Data Gallery

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  WB analysis of SKOV-3 using 25471-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25471-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, A549 cells, COLO 320 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25471-1-AP is STK10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK10 fusion protein Ag22169
• Full Name: serine/threonine kinase 10
• Calculated Molecular Weight: 968 aa, 112 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC070077
• Gene Symbol: STK10
• Gene ID (NCBI): 6793
• RRID: AB_2880096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressedas a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

Publications

Species	Application	Title
		Clin Cancer Res; Combined CDK4/6 and ERK1/2 inhibition enhances anti-tumor activity in NF1-associated plexiform neurofibroma; Authors - Alyssa C Flint

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jared (Verified Customer) (08-21-2019)

This antibody is an all-star antibody. I can even re use it a couple of times, and it still works. potent stuff. Picture would not upload... There is one strong band, and it shows virtually nothing in the lane where I CRISPR'd out LOK aka STK10 in the LOK band area. There is a non-specific band, but that is located clearly out of the range of where the LOK band is located. Non-specific band at approx 200kd. LOK for me runs at around 140kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: 293T and H1299

STK10 Antibody Western Blot, validation (1:3000 dilution) in 293T and H1299 (Cat no:25471-1-AP)