STARD13 Polyclonal antibody

STARD13 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21325-1-AP

Synonyms

46H23.2, DLC 2, DLC2, GT650, Rho GTPase activating protein, STARD13

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Validation Data Gallery

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  WB analysis of MCF-7 using 21325-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21325-1-AP (STARD13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21325-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21325-1-AP (STARD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 21325-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 21325-1-AP (STARD13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 21325-1-AP is STARD13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STARD13 fusion protein Ag15012
• Full Name: StAR-related lipid transfer (START) domain containing 13
• Calculated Molecular Weight: 1113 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC046563
• Gene Symbol: STARD13
• Gene ID (NCBI): 90627
• RRID: AB_2878840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,WB	Endocrine; Loss of STARD13 contributes to aggressive phenotype transformation and poor prognosis in papillary thyroid carcinoma; Authors - Chuimian Zeng ; KD Validated
human	WB	Life Sci; Role of exosomal microRNA-125b-5p in conferring the metastatic phenotype among pancreatic cancer cells with different potential of metastasis.; Authors - Mengwei Wu
rat	WB	Environ Toxicol; MiR-125b enhances doxorubicin-induced cardiotoxicity by suppressing the nucleus-cytoplasmic translocation of YAP via targeting STARD13.; Authors - Xiaoping Jin
human,mouse	WB,IHC	Biol Proced Online; Circ_0003570 Suppresses the progression of hepatocellular carcinoma through miR-182-5p/STARD13 regulatory axis; Authors - Xu Zhang