CoraLite®594-conjugated ST6GALNAC6 Monoclonal antibody

ST6GALNAC6 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A7B7

Cat no : CL594-66209

Synonyms

hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

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Validation Data Gallery

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  IF Staining of human colon cancer using CL594-66209

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 ST6GALNAC6 antibody (CL594-66209, Clone: 1A7B7 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human colon cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66209 targets ST6GALNAC6 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ST6GALNAC6 fusion protein Ag8358
• Full Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
• Calculated Molecular Weight: 333aa,38 kDa; 374aa,41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC007802
• Gene Symbol: ST6GALNAC6
• Gene ID (NCBI): 30815
• RRID: AB_2919965
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.