ST6GAL1 Monoclonal antibody

ST6GAL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F11G5

Cat no : 68633-1-Ig

Synonyms

Alpha 2,6 ST 1, B cell antigen CD75, CD75, Sialyltransferase 1, SIAT1, ST6Gal I, ST6GAL1, ST6GalI, ST6N

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Validation Data Gallery

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  WB analysis of TF-1 using 68633-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 68633-1-Ig (ST6GAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68633-1-Ig targets ST6GAL1 in WB, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ST6GAL1 fusion protein Ag6181
• Full Name: ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 43-45 kDa, 50-70 kDa
• GenBank Accession Number: BC040009
• Gene Symbol: ST6GAL1
• Gene ID (NCBI): 6480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with unknown reason. (PMID: 15049997)