SSX4 Polyclonal antibody

SSX4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15876-1-AP

Synonyms

Cancer/testis antigen 5.4, CT5.4, Protein SSX4, SSX4, SSX4A, SSX4B



Tested Applications

Positive WB detected inMCF7 cells, A549 cells, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15876-1-AP targets SSX4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSX4 fusion protein Ag8637
Full Name synovial sarcoma, X breakpoint 4
Calculated Molecular Weight 188 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005325
Gene Symbol SSX4
Gene ID (NCBI) 6759
RRIDAB_2035018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SSX4 antibody 15876-1-APDownload protocol
IHC protocol for SSX4 antibody 15876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols