SSRP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SSRP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15696-1-AP

Synonyms

FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160

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Validation Data Gallery

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  WB analysis of HeLa using 15696-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15696-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15696-1-AP (SSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15696-1-AP

  IP result of anti-SSRP1 (IP:15696-1-AP, 4ug; Detection:15696-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of human ovary tumor using 15696-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 15696-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15696-1-AP (SSRP1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 15696-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15696-1-AP (SSRP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Product Information

15696-1-AP targets SSRP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSRP1 fusion protein Ag8318
• Full Name: structure specific recognition protein 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC005116
• Gene Symbol: SSRP1
• Gene ID (NCBI): 6749
• RRID: AB_2195756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma.; Authors - Daniel R Carter
human	WB	ACS Appl Mater Interfaces; Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.; Authors - Chenyan Yu ; KD Validated
mouse	IHC	FASEB J; Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.; Authors - Ikumi Ohsawa
human	WB,IHC	Int J Med Sci; SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.; Authors - Qian Wang ; KD Validated
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	WB,IHC	J Cell Mol Med; SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.; Authors - Wei Wu ; KD Validated