SRP9 Monoclonal antibody

• Featured Product
• KD/KO Validated

SRP9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H7G10

Cat no : 66068-1-Ig

Synonyms

ALURBP, SRP9

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Validation Data Gallery

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  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66068-1-Ig

  WB result of SRP9 antibody (66068-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP9 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 66068-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66068-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66068-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HeLa cells, HEK-293 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66068-1-Ig targets SRP9 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SRP9 fusion protein Ag17153
• Full Name: signal recognition particle 9kDa
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC015094
• Gene Symbol: SRP9
• Gene ID (NCBI): 6726
• RRID: AB_11042618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.