SRGAP1 Polyclonal antibody

SRGAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13252-1-AP

Synonyms

ARHGAP13, KIAA1304, SRGAP1

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Validation Data Gallery

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  WB analysis using 13252-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13252-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13252-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13252-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13252-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 13252-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 13252-1-AP (SRGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13252-1-AP

  IP result of anti-SRGAP1 (IP:13252-1-AP, 4ug; Detection:13252-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13252-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13252-1-AP (SRGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, Apoptosised HeLa cells, mouse kidney tissue, HEK-293 cells, SH-SY5Y cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue, mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

13252-1-AP targets SRGAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRGAP1 fusion protein Ag4104
• Full Name: SLIT-ROBO Rho GTPase activating protein 1
• Calculated Molecular Weight: 1085 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC029919
• Gene Symbol: SRGAP1
• Gene ID (NCBI): 57522
• RRID: AB_2270916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	J Am Soc Nephrol; SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance.; Authors - Manuel Rogg
human	WB	J Exp Clin Cancer Res; srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer.; Authors - Yuyang Feng
human	WB,IF	Cell Rep; A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling; Authors - Chandrani Mondal