SQRDL Monoclonal antibody

SQRDL Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B4E3

Cat no : 68459-1-Ig

Synonyms

CGI 44, SQRDL

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Validation Data Gallery

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  WB analysis using 68459-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68459-1-Ig (SQRDL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 68459-1-Ig

  IP result of anti-SQRDL (IP:68459-1-Ig, 4ug; Detection:68459-1-Ig 1:3000) with HeLa cells lysate 1560 ug.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, HT-29 cells, hTERT-RPE1 cells, THP-1 cells, pig liver tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68459-1-Ig targets SQRDL in WB, IP, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SQRDL fusion protein Ag10762
• Full Name: sulfide quinone reductase-like (yeast)
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC016836
• Gene Symbol: SQRDL
• Gene ID (NCBI): 58472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.