PBK Polyclonal antibody

• Featured Product
• KD/KO Validated

PBK Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 16110-1-AP

Synonyms

Cancer/testis antigen 84, CT84, MAPKK like protein kinase, Nori 3, PBK, PDZ binding kinase, SPK, TOPK

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Validation Data Gallery

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  WB analysis using 16110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16110-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16110-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16110-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HepG2 using 16110-1-AP

  (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse ovary tissue, mouse testis tissue, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human placenta tissue, human lung cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

16110-1-AP targets PBK in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBK fusion protein Ag9033
• Full Name: PDZ binding kinase
• Calculated Molecular Weight: 322 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC015191
• Gene Symbol: PBK
• Gene ID (NCBI): 55872
• RRID: AB_2283508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Publications

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun
mouse	WB,IF	FASEB J; Autophagy plays a positive role in induction of epidermal proliferation.; Authors - Junyi Wang
mouse	IF	Invest Ophthalmol Vis Sci; Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea; Authors - Yueh-Feng Wu
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
mouse	WB,IF	Invest Ophthalmol Vis Sci; Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.; Authors - Nihal Kaplan ; KD Validated
rat	WB	Front Endocrinol (Lausanne); PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas.; Authors - Kejing Zhu