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  • KD/KO Validated

SPG11 Polyclonal antibody

SPG11 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16555-1-AP

Synonyms

KIAA1840, Spastic paraplegia 11 protein, SPATACSIN, SPG11



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16555-1-AP targets SPG11 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPG11 fusion protein Ag9769
Full Name spastic paraplegia 11 (autosomal recessive)
Calculated Molecular Weight 2443 aa, 279 kDa
GenBank Accession NumberBC024161
Gene Symbol SPG11
Gene ID (NCBI) 80208
RRIDAB_2878277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SPG11 antibody 16555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

NUFIP1 is a ribosome receptor for starvation-induced ribophagy.

Authors - Gregory A Wyant
humanWB

J Clin Invest

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

Authors - Jaerak Chang
mouseWB

PLoS Genet

A hereditary spastic paraplegia mouse model supports a role of ZFYVE26/SPASTIZIN for the endolysosomal system.

Authors - Mukhran Khundadze
humanWB

Hum Mol Genet

SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction.

Authors - Lara Marrone
mouseWB

Neurobiol Dis

Loss of spatacsin function alters lysosomal lipid clearance leading to upper and lower motor neuron degeneration.

Authors - Julien Branchu
  • KO Validated
humanWB

Ann Clin Transl Neurol

Lysosomal abnormalities in hereditary spastic paraplegia types SPG15 and SPG11.

Authors - Benoît Renvoisé
  • KD Validated