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CoraLite® Plus 488-conjugated SOX10 Monoclonal antibody

SOX10 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C8

Cat no : CL488-66786

Synonyms

DOM, SOX10, Transcription factor SOX 10, WS2E, WS4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    IF Staining of mouse brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    IF Staining of rat brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C6 cells using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    FC experiment of C6 using CL488-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SOX10 (CL488-66786, Clone:1D2C8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inmouse brain tissue, rat brain tissue
Positive FC detected inC6 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66786 targets SOX10 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SOX10 fusion protein Ag23191
Full Name SRY (sex determining region Y)-box 10
Calculated Molecular Weight 50 kDa
GenBank Accession NumberBC002824
Gene Symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2919369
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
FC protocol for CL Plus 488 SOX10 antibody CL488-66786Download protocol
Standard Protocols
Click here to view our Standard Protocols