CoraLite® Plus 488-conjugated SNX9 Monoclonal antibody

SNX9 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11C9

Cat no : CL488-68255

Synonyms

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP

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Validation Data Gallery

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  IF Staining of A431 using CL488-68255

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 SNX9 antibody (CL488-68255, Clone: 1B11C9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68255 targets SNX9 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNX9 fusion protein Ag8446
• Full Name: sorting nexin 9
• Calculated Molecular Weight: 595 aa, 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC005022
• Gene Symbol: SNX9
• Gene ID (NCBI): 51429
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).