SNX19 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNX19 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13410-1-AP

Synonyms

CHET8, KIAA0254, SNX19, sorting nexin 19

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Validation Data Gallery

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  WB analysis using 13410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13410-1-AP (SNX19 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13410-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13410-1-AP (SNX19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13410-1-AP

  WB result of SNX19 antibody (13410-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX19 transfected HeLa cells.

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  WB analysis of K-562 using 13410-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13410-1-AP (SNX19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13410-1-AP

  IP result of anti-SNX19 (IP:13410-1-AP, 4ug; Detection:13410-1-AP 1:500) with Raji cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: Raji cells, HeLa cells, K-562 cells
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13410-1-AP targets SNX19 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX19 fusion protein Ag4059
• Full Name: sorting nexin 19
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 109-110 kDa, 130 kDa
• GenBank Accession Number: BC031620
• Gene Symbol: SNX19
• Gene ID (NCBI): 399979
• RRID: AB_2192527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	FASEB J; PMID: 32259353; Authors - Andrew C Tiu