SMYD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMYD2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 21290-1-AP

Synonyms

HSKM B, KMT3C, Lysine N methyltransferase 3C, SMYD2, ZMYND14

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Validation Data Gallery

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  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMYD2 antibody (21290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C2C12 cell, HEK-293 cells, C2C12 cells, U2OS cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 2 publications below

Product Information

21290-1-AP targets SMYD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMYD2 fusion protein Ag15823
• Full Name: SET and MYND domain containing 2
• Calculated Molecular Weight: 433 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC098305
• Gene Symbol: SMYD2
• Gene ID (NCBI): 56950
• RRID: AB_10733881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMYD2, also known as SET and MYND domain containing 2, encodes an enzyme called N-lysine methyltransferase SMYD2, which plays a crucial role in cellular processes, including histone methylation, non-histone protein methylation, cell proliferation and differentiation, DNA repair (PMID: 18065756, PMID: 36191822). Aberrant SMYD2 expression has been linked to various cancers, including colon cancer, lung cancer, and cervical cancer. Smyd2 is strongly expressed in muscle cells with highest expression in the neonatal heart (PMID: 25125178).

Publications

Species	Application	Title
mouse	IHC	Cell Death Dis; SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth.; Authors - Yu-Qiang Yu
human	IHC	Theranostics; Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma.; Authors - Libin Yan ; KD Validated
human	IHC	Oncogene; Lysine methyltransferase SMYD2 enhances androgen receptor signaling to modulate CRPC cell resistance to enzalutamide; Authors - Junhong Li
human	WB	Stem Cells; SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells through Mediating the Transcriptional Activation of Key Mesendodermal Genes.; Authors - Hua-Jun Bai ; KO Validated
human	WB,IHC	Am J Cancer Res; SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.; Authors - Fanchao Meng ; KD Validated
mouse	WB,IHC,IF	J Pharmacol Sci; Pharmacological inhibition of SMYD2 protects against cisplatin-induced renal fibrosis and inflammation; Authors - Min Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

This antibody worked well in the differentiated human cardiomyocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human cardiomyocytes