SMPX Polyclonal antibody

SMPX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15791-1-AP

Synonyms

small muscle protein, X linked, Small muscular protein, SMPX, SRMX

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 15791-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15791-1-AP

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 15791-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15791-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: pig skeletal muscle tissue, human skeletal muscle tissue, human colon tissue, pig colon tissue
• Positive IHC detected in: human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15791-1-AP targets SMPX in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMPX fusion protein Ag8497
• Full Name: small muscle protein, X-linked
• Calculated Molecular Weight: 88 aa, 10 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC005948
• Gene Symbol: SMPX
• Gene ID (NCBI): 23676
• RRID: AB_2193527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.