SMC5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMC5 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 14178-1-AP

Synonyms

hSMC5, KIAA0594, SMC 5, SMC protein 5, SMC5, SMC5L1

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Validation Data Gallery

• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14178-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14178-1-AP (SMC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14178-1-AP targets SMC5 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMC5 fusion protein Ag5385
• Full Name: structural maintenance of chromosomes 5
• Calculated Molecular Weight: 1101 aa, 129 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC038225
• Gene Symbol: SMC5
• Gene ID (NCBI): 23137
• RRID: AB_2192775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas ; KD Validated
human	IF	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-30-2022)

The detection of SMC5 by WB is fair with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2 cell

SMC5 Antibody Western Blot validation (1/1000 dilution) in HepG2 cell (Cat no:14178-1-AP)