SMC1A Polyclonal antibody

SMC1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21695-1-AP

Synonyms

CDLS2, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB

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Validation Data Gallery

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  WB analysis using 21695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21695-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21695-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21695-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21695-1-AP (SMC1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 21695-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 21695-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21695-1-AP (SMC1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

21695-1-AP targets SMC1A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMC1A fusion protein Ag16286
• Full Name: structural maintenance of chromosomes 1A
• Calculated Molecular Weight: 1233 aa, 143 kDa
• Observed Molecular Weight: 143-150 kDa
• GenBank Accession Number: BC112127
• Gene Symbol: SMC1A
• Gene ID (NCBI): 8243
• RRID: AB_2878904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Publications

Species	Application	Title
human	WB	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos
human	WB,IHC	Cancer Med; PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A; Authors - Weidong Lin
human	WB	Front Genet; In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.; Authors - Wen-Juan Xiao