SMARCD1 Monoclonal antibody

SMARCD1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F2G6

Cat no : 66116-1-Ig

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

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Validation Data Gallery

• 

  WB analysis of Jurkat using 66116-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66116-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66116-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66116-1-Ig (SMARCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66116-1-Ig is SMARCD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SMARCD1 fusion protein Ag19100
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC009368
• Gene Symbol: SMARCD1
• Gene ID (NCBI): 6602
• RRID: AB_2881515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.