SMARCD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMARCD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10998-2-AP

Synonyms

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

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Validation Data Gallery

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  WB analysis of mouse brain using 10998-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10998-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10998-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10998-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10998-2-AP

  IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lymphoma using 10998-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 10998-2-AP

  Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10998-2-AP targets SMARCD1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCD1 fusion protein Ag1453
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC009368
• Gene Symbol: SMARCD1
• Gene ID (NCBI): 6602
• RRID: AB_2192133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; BAF60a deficiency uncouples chromatin accessibility and cold sensitivity from white fat browning.; Authors - Tongyu Liu ; KO Validated
mouse	CoIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu
human	WB	Cancer Lett; MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression.; Authors - Shuo Chen
human	WB	J Biol Chem; Phosphorylation of Williams syndrome transcription factor by MAPK induces a switching between two distinct chromatin remodeling complexes.; Authors - Oya Hiroyuki H
human	WB	J Cell Mol Med; DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression.; Authors - Li-Li Wang
human	WB	NPJ Aging Mech Dis; SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes.; Authors - Chisato Inoue ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marian (Verified Customer) (11-13-2019)

We used SMARCD1 Antibody to perform microwestern arrays. We obtained very precise and biologically relevant results. The blots were clean and smear-free.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000