SMARCAL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMARCAL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12513-1-AP

Synonyms

HARP, HepA related protein, HHARP, SMARCAL1

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12513-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12513-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12513-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

12513-1-AP targets SMARCAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCAL1 fusion protein Ag3194
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
• Calculated Molecular Weight: 954 aa, 106 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: BC016482
• Gene Symbol: SMARCAL1
• Gene ID (NCBI): 50485
• RRID: AB_2191690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCAL1 is identified as a factor that favors tumor immune evasion by a dual mechanism involving both the suppression of innate immune signaling and the induction of PD-L1-mediated immune checkpoint responses. Mechanistically, SMARCAL1 limits endogenous DNA damage, thereby suppressing cGAS-STING-dependent signaling during cancer cell growth. Simultaneously, it cooperates with the AP-1 family member JUN to maintain chromatin accessibility at a PD-L1 transcriptional regulatory element, thereby promoting PD-L1 expression in cancer cells (PMID: 38301646).

Publications

Species	Application	Title
human	WB,IP	Sci Total Environ; LncRNA MT1DP promotes cadmium-induced DNA replication stress by inhibiting chromatin recruitment of SMARCAL1.; Authors - Wenya Feng
human	WB	J Exp Clin Cancer Res; RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma; Authors - Qiu Tu ; KD Validated