SMARCA4/BRG1 Polyclonal antibody

SMARCA4/BRG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20650-1-AP

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

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Validation Data Gallery

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  WB analysis of HepG2 using 20650-1-AP

  WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

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  WB analysis of HeLa using 20650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

20650-1-AP targets SMARCA4/BRG1 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Calculated Molecular Weight: 185 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_001128844
• Gene Symbol: SMARCA4
• Gene ID (NCBI): 6597
• RRID: AB_10694827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.

Publications

Species	Application	Title
mouse	WB,IF,ChIP	Acta Pharmacol Sin; Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction; Authors - Jing Li