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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SMARCA2-Specific Polyclonal antibody

SMARCA2-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26613

Synonyms

ATP dependent helicase SMARCA2, BAF190, BAF190B, BRG1 associated factor 190B, BRM, FLJ36757, hBRM, hSNF2a, Protein brahma homolog, SMARCA2, SNF2, SNF2 alpha, SNF2A, SNF2L2, SNF2LA, Sth1p, SWI2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SMARCA2-Specific Pol (CL488-26613)

    FC experiment of HeLa using CL488-26613

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SMARCA2-Specific (CL488-26613) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-26613 targets SMARCA2-Specific in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCA2-Specific fusion protein Ag25157
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Calculated Molecular Weight 181 kDa
Observed Molecular Weight 180-190 kDa
GenBank Accession NumberNM_003070
Gene Symbol SMARCA2
Gene ID (NCBI) 6595
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.