SLC7A5 Monoclonal antibody

SLC7A5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2G5H3

Cat no : 67951-1-Ig

Synonyms

4F2 LC, 4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5

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Validation Data Gallery

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  WB analysis using 67951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67951-1-Ig (SLC7A5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67951-1-Ig targets SLC7A5 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC7A5 fusion protein Ag20291
• Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 35-50 kDa
• GenBank Accession Number: BC039692
• Gene Symbol: SLC7A5
• Gene ID (NCBI): 8140
• RRID: AB_2918703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Metab; Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness; Authors - Hongcheng Cheng
		J Med Chem; Discovery of Novel Aminobutanoic Acid-Based ASCT2 Inhibitors for the Treatment of Non-Small-Cell Lung Cancer; Authors - Lian Qin